基于网络药理学的小承气汤治疗功能性便秘作用机制研究
投稿时间:2020-03-06     点此下载全文
引用本文:阮庆婷,张羽,黄美祯,潘春曲,谢胜.基于网络药理学的小承气汤治疗功能性便秘作用机制研究[J].中国现代中药,2020,22(12):2006-2014
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作者中文名作者英文名单位中文名单位英文名E-Mail
阮庆婷 RUAN Qing-ting 广西中医药大学 研究生院,广西南宁530001 Graduate School of Guangxi University of Traditional Chinese Medicine,Nanning 530001,China  
张羽 ZHANG Yu 广西中医药大学 研究生院,广西南宁530001 Graduate School of Guangxi University of Traditional Chinese Medicine,Nanning 530001,China  
黄美祯 HUANG Mei-zhen 广西中医药大学 研究生院,广西南宁530001 Graduate School of Guangxi University of Traditional Chinese Medicine,Nanning 530001,China  
潘春曲 PAN Chun-qu 广西中医药大学 研究生院,广西南宁530001 Graduate School of Guangxi University of Traditional Chinese Medicine,Nanning 530001,China  
谢胜 XIE Sheng 广西中医药大学 第一附属医院,广西南宁530000 The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530000,China 谢胜,教授,博士研究生导师,主任医师,研究方向:脾胃四时调四脏防治疾病研究;E-mail:xiesheng2018tougao@163.com 
基金项目:广西科技基地和人才专项(桂科AD1850005);广西中医药大学2020年研究生教育创新计划项目(YCSY2020024)
中文摘要:目的:利用网络药理学方法探讨小承气汤治疗功能性便秘的作用靶点,并对其进行生物信息学分析。方法:应用中药系统药理学技术平台(TCMSP)数据库筛选出小承气汤中的潜在化学活性物质及相关靶点蛋白;利用GeneCards和OMIM数据库筛选功能性便秘疾病作用相关靶点,并运用Uniprot数据库查询与之相对应的基因名称;借助Cytoscape 3.7.2软件,构建“药物-疾病-靶点”的可视化网络图,通过STRING数据库平台构建蛋白相互作用网络,再利用Bioconductor平台和R语言进行基因本体(GO)富集分析和京都基因与基因组百科全书(KEGG)富集分析。结果:通过筛选,共获得小承气汤中的化学成分40个,与功能性便秘相关的靶点4556个,小承气汤-功能性便秘的共同靶点99个;核心靶点涉及丝/苏氨酸蛋白激酶1(AKT1)、丝裂原活化蛋白激酶3(MAPK3)、白细胞介素-6(IL-6)、胱天蛋白酶-3(CASP3)等;获得108条GO生物学过程,通过KEGG分析得出富集通路158条。结论:基于网络药理学分析得出小承气汤有效化学成分,初步预测了小承气汤中的化学成分是通过AKT1、MAPK3、IL-6等靶点及乙型肝炎、磷脂酰肌醇3-激酶/蛋白激酶B、前列腺癌、内分泌抵抗等信号通路发挥治疗功能性便秘的作用,为其的临床应用提供了理论依据。
中文关键词:小承气汤  功能性便秘  网络药理学  机制
 
Mechanism of Xiaochengqi Decoction in Treating Functional Constipation Based on Network Pharmacology
Abstract:Objective:To explore the role of Xiaochengqi Decoction in treating functional constipation by using network pharmacology,and to analyze it by bioinformatics. Methods:The Chinese medicine system pharmacology technology platform (TCMSP) database was used to screen potential chemically active substances and related target proteins in Xiaochengqi Decoction. GeneCards and OMIM databases were used to screen targets related to the function of functional constipation diseases. The Uniprot database was used query the corresponding gene name,use Cytoscape 3.7.2 software to build a ″drug-disease-target″ network diagram visualization,build a protein interaction network through the STRING database platform,and then use the Bioconductor platform and R language to perform GO enrichment analysis and KEGG enrichment analysis. Results:Through screening,a total of 40 chemical substances of Xiaochengqi Decoction were obtained,4556 targets related to functional constipation,and 99 common targets of Xiaochengqi Decoction-functional constipation were selected. The core targets include serine/threonine protein kinase 1 (AKT1),mitogen activated protein kinase 3 (MAPK3),interleukin-6 (IL-6),Caspases-3 (CASP3),etc. A total of 108 GO biological processes were obtained,and 158 enrichment pathways were obtained by KEGG analysis. Conclusion:The effective chemical components of Xiaochengqi Decoction are obtained based on network pharmacological analysis,and it is preliminarily predicted that the chemical components of Xiaochengqi decoction play a role in the treatment of functional constipation through targets such as AKT1,MAPK3,IL-6 and signal pathways such as hepatitis B,phosphatidylinositol 3-kinase/protein kinase B,prostate cancer and endocrine resistance,which provides a theoretical basis for its clinical application.
keywords:Xiaochengqi Decoction  functional constipation  network pharmacology  mechanism
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