青钱柳叶质量控制方法研究
投稿时间:2020-04-07     点此下载全文
引用本文:毛菊华,余华丽,陈张金,范蕾,吴查青.青钱柳叶质量控制方法研究[J].中国现代中药,2021,23(3):453-456
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作者中文名作者英文名单位中文名单位英文名E-Mail
毛菊华 MAO Ju-hua 丽水市质量检验检测研究院,浙江丽水323000 Lishui Institute for Quality Inspection and Testing,Lishui 323000,China  
余华丽 YU Hua-li 丽水市质量检验检测研究院,浙江丽水323000 Lishui Institute for Quality Inspection and Testing,Lishui 323000,China 余华丽,副主任中药师,研究方向:中药与民族药质量研究;Tel:(0578)2185211,E-mail:516739410@qq.com 
陈张金 CHEN Zhang-jin 丽水市质量检验检测研究院,浙江丽水323000 Lishui Institute for Quality Inspection and Testing,Lishui 323000,China  
范蕾 FAN Lei 丽水市质量检验检测研究院,浙江丽水323000 Lishui Institute for Quality Inspection and Testing,Lishui 323000,China  
吴查青 WU Cha-qing 丽水市质量检验检测研究院,浙江丽水323000 Lishui Institute for Quality Inspection and Testing,Lishui 323000,China  
基金项目:丽水市科技项目(2017GYX47)
中文摘要:目的:建立青钱柳叶的质量控制方法。方法:运用显微鉴别法、薄层色谱法、超高效液相色谱法分别对青钱柳叶的显微特征、指标性成分(槲皮素、山柰酚、绿原酸、异槲皮苷和槲皮苷)进行定性鉴别和定量测定。结果:各批次样品显微特征及薄层色谱特征明显,青钱柳叶中绿原酸、异槲皮苷、槲皮苷质量分数分别为0.05%~1.79%、0.30%~1.00%、0.11%~0.74%。结论:建立的质量控制方法简便、准确、重复性好,可为该药材的质量标准建立提供参考。
中文关键词:青钱柳叶  质量控制  薄层色谱  超高效液相色谱法
 
Study on Quality Control of Cyclocaryae Folium
Abstract:Objective:To establish a method for quality control of Cyclocaryae Folium.Methods:With the methods of microscopical identification,thin-layer chromatography (TLC) and ultra performance liquid chromatography (UPLC) to identify or determine its microscopic chatacteristics and index ingredients (quercetin,kaempferol,chlorogenic acid,isoquercitrin and quercitrin). Results:The characteristics of microscopic identification and TLC of Cyclocaryae Folium were obviously;the content of chlorogenic acid,isoquercitrin,quercitrin were in the range of 0.05%-1.79%,0.30%-1.00%,0.11%-0.74%,respectively. Conclusion:This method is simple,accurate and reproducible,which can provide theoretical basis for setting the quality standard of Cyclocaryae Folium.
keywords:Cyclocaryae Folium  quality control  TLC  UPLC
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