| 基于HPLC多成分定量结合外观性状的金银花质量评价与等级标准的制定 |
| 投稿时间：2021-06-30 点此下载全文
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| 引用本文：代熙,郭然,黄厚钰,耿泽宇,袁皓,翟玉生,郝恩普,李卫东.基于HPLC多成分定量结合外观性状的金银花质量评价与等级标准的制定[J].中国现代中药,2022,24(5):821-830 |
| DOI：10.13313/j.issn.1673-4890.20210630007 |
| 摘要点击次数: 251 |
| 全文下载次数: 40 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 代熙 |
DAI Xi |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 郭然 |
GUO Ran |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 黄厚钰 |
HUANG Hou-yu |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 耿泽宇 |
GENG Ze-yu |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 袁皓 |
YUAN Hao |
山东坤和堂药业股份有限公司，山东 菏泽 274000 |
Shandong Kunhetang Pharmaceutical Co., Ltd., Heze 274000, China |
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| 翟玉生 |
ZHAI Yu-sheng |
通渭县清凉沅金银花产业扶贫开发有限公司，甘肃 通渭 743300 |
Qingliang Yuan Honeysuckle Industry Poverty Alleviation Development Co., Ltd. in Tongwei, Tongwei 743300, China |
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| 郝恩普 |
HAO En-pu |
巨鹿县发展和改革局，河北 巨鹿 055250 |
Development and Reform Bureau (Science and Industry) of Julu County, Julu 055250, China |
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| 李卫东* |
LI Wei-dong |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 基金项目:国家自然科学基金项目（31370360）；2021年度山东省重点扶持区域引进急需紧缺人才项目 |
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| 中文摘要:目的 分析对比主产区和部分新产区金银花的差异，研究金银花药材质量的分级规律，建立广泛通用的金银花等级质量标准。方法 收集8个地区共计78批样品，测定外观性状指标，包括颜色、黄条率和枝叶率，通过高效液相色谱法测定绿原酸、异绿原酸A、异绿原酸C、总酚酸和木犀草苷含量；使用SPSS 26.0和SIMCA 14.1软件进行质量评价和等级划分。结果 8个地区金银花样品绿原酸质量分数为1.17%～3.66%，总酚酸质量分数为3.34%～6.59%，木犀草苷质量分数为0.045%～0.185%。主成分分析对比发现甘肃通渭、云南云龙地区金银花同主产区金银花存在较大差异，不同加工方式处理的金银花区分较为明显。根据综合评分结果将金银花分为4个等级。结论 部分样品不合格的主要原因为总酚酸含量不达标。主产区金银花特征相似，不同新产区的金银花质量差异较大。建立的金银花等级质量标准能够反映药材质量的差异性，为不同产地金银花药材质量评价提供参考。 |
| 中文关键词:金银花 绿原酸 异绿原酸A 异绿原酸C 木犀草苷 质量标准 |
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| Quality Evaluation and Grading Standard of Lonicera Japonica Flos Based on HPLC Multi-Component Quantitative Analysis and Appearance Traits |
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| Abstract:Objective To analyze and compare the differences of Lonicerae Japonicae Flos (LJF) between the major and new producing regions and explore the pattern of quality grading of LJF, thereby establishing a widely-used quality standard for LJF grading.Methods Seventy-eight samples of LJF from 8 different regions were collected to determine the indexes of appearance traits, including color, yellow strip rate, and rate of branch and leaf. Chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C, total phenolic acid content, and luteoloside content were determined by high performance liquid chromatograph (HPLC). SPSS 26.0 and SIMCA 14.1 were used to evaluate the quality and classify the grades.Results The chlorogenic acid content, the total phenolic acid content, and the content of luteoloside of the samples in the 8 producing regions were 1.17%-3.66%, 3.34%-6.59%, and 0.045%-0.185%, respectively. Principal component analysis showed that LJF in Tongwei, Gansu province and Yunlong, Yunnan province were significantly different from that in the main producing regions, and LJF treated by different processing methods varied obviously. LJF was divided into four grades based on the comprehensive scoring results.Conclusion The main reason for the unqualified LJF samples was that the content of total phenolic acids did not meet the standard. The characteristics of LJF in the major producing regions were similar, while the quality of LJF in the new producing regions was significantly different. The established quality standards for LJF grading reflected the quality differences of LJF and provided references for the quality evaluation of LJF in different producing regions. |
| keywords:Lonicerae Japonicae Flos chlorogenic acid isochlorogenic acid A isochlorogenic acid C luteoloside quality standard |
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