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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
傅咏梅	FU Yong-mei	国药集团广东环球制药有限公司，广东佛山 528303	Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd., Foshan 528303, China
卢伟玲	LU Wei-ling	国药集团广东环球制药有限公司，广东佛山 528303	Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd., Foshan 528303, China
王欢*	WANG Huan	国药集团广东环球制药有限公司，广东佛山 528303	Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd., Foshan 528303, China
黄后楷	HUANG Hou-kai	国药集团广东环球制药有限公司，广东佛山 528303	Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd., Foshan 528303, China
陈静	CHEN Jing	国药集团广东环球制药有限公司，广东佛山 528303	Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd., Foshan 528303, China
车轸潮	CHE Zhen-chao	国药集团广东环球制药有限公司，广东佛山 528303	Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd., Foshan 528303, China
罗锦波	LUO Jin-bo	国药集团广东环球制药有限公司，广东佛山 528303	Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd., Foshan 528303, China
梁砚康	LIANG Yan-kang	国药集团广东环球制药有限公司，广东佛山 528303	Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd., Foshan 528303, China

基金项目:工业和信息化部2022年产业技术基础公共服务平台项目（2022-230-221）

中文摘要:目的建立复方罗布麻颗粒的超高效液相色谱法（UPLC）特征图谱，同时对新绿原酸、绿原酸、隐绿原酸、金丝桃苷、异槲皮苷、木犀草苷进行含量测定。方法以乙腈-0.1%磷酸水溶液为流动相，梯度洗脱，色谱柱为Waters CORTECS T3（100 mm×2.1 mm，1.6 μm），流速为0.33 mL·min^–1，检测波长为360 nm，柱温为30 ℃。结果得到15批复方罗布麻颗粒特征图谱，确定特征峰14个，相似度值均大于0.95。新绿原酸、绿原酸、隐绿原酸、金丝桃苷、异槲皮苷、木犀草苷分别在相应质量浓度范围内与其峰面积呈良好线性关系（r≥0.999 9）；平均回收率分别为99.9%、100.8%、99.9%、97.6%、101.1%、100.6%，RSD均小于2.0%。结论该方法具有较高的灵敏度、良好的重复性，可为科学、全面地评价和控制复方罗布麻颗粒的质量提供参考。

中文关键词:复方罗布麻颗粒含量测定特征图谱

UPLC Specific Chromatogram of Compound Luobuma Granules and Content Determination of Six Components

Abstract:Objective To establish a ultra performance liquid chromatography (UPLC) for Compound Luobuma Granules, and neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, hyperoside, isoquercitrin and cynaroside were determined.Methods Gradient elution was used with Waters CORTECS T3 (100 mm×2.1 mm, 1.6 μm) column and acetonitrile-0.1% phosphoric acid as mobile phase at a flow rate of 0.33 mL·min^-1. The detection wavelength was 360 nm, and column temperature was 30 ℃.Results The specific chromatogram of 15 batches of Compound Luobuma Granules were established, from which 14 characteristic peaks were identified, with all the similarities more than 0.95. Neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, hyperoside, isoquercitrin and cynaroside all had good linear relationships with the peak area in their corresponding ranges (r≥0.999 9). The average recoveries of the six components were 99.9%, 100.8%, 99.9%, 97.6%, 101.1%, and 100.6%, respectively, with RSD<2.0%.Conclusion This developed UPLC had high sensitivity and good reproducibility, which provided a basis for scientific evaluation and comprehensive control of the quality of Compound Luobuma Granules.

keywords:Compound Luobuma Granules content determination specific chromatogram

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