| 基于UHPLC-Q-Exactive Orbitrap MS的复方一枝蒿颗粒化学成分快速鉴定 |
| 投稿时间：2023-03-03 点此下载全文
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| 引用本文：刘明娟,李凯琳,张敏,唐苏女,刘玲,李晴,郭俐兰,杨建波,李辉.基于UHPLC-Q-Exactive Orbitrap MS的复方一枝蒿颗粒化学成分快速鉴定[J].中国现代中药,2024,26(3):507-517 |
| DOI：10.13313/j.issn.1673-4890.20230303002 |
| 摘要点击次数: 102 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 刘明娟 |
LIU Ming-juan |
湖南医药学院 药学院 侗医药研究湖南省重点实验室，湖南 怀化 418000 |
School of Pharmaceutical Sciences, Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua 418000, China |
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| 李凯琳 |
LI Kai-lin |
湖南医药学院 药学院 侗医药研究湖南省重点实验室，湖南 怀化 418000 |
School of Pharmaceutical Sciences, Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua 418000, China |
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| 张敏 |
ZHANG Min |
湖南医药学院 药学院 侗医药研究湖南省重点实验室，湖南 怀化 418000 |
School of Pharmaceutical Sciences, Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua 418000, China |
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| 唐苏女 |
TANG Su-nyu |
湖南医药学院 药学院 侗医药研究湖南省重点实验室，湖南 怀化 418000 |
School of Pharmaceutical Sciences, Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua 418000, China |
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| 刘玲 |
LIU Ling |
湖南医药学院 药学院 侗医药研究湖南省重点实验室，湖南 怀化 418000 |
School of Pharmaceutical Sciences, Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua 418000, China |
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| 李晴 |
LI Qing |
湖南医药学院 药学院 侗医药研究湖南省重点实验室，湖南 怀化 418000 |
School of Pharmaceutical Sciences, Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua 418000, China |
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| 郭俐兰 |
GUO Li-lan |
湖南医药学院 药学院 侗医药研究湖南省重点实验室，湖南 怀化 418000 |
School of Pharmaceutical Sciences, Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua 418000, China |
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| 杨建波* |
YANG Jian-bo |
新疆维吾尔自治区药品检验研究院 国家药品监督管理局中药（维药）质量控制重点实验室， 新疆 乌鲁木齐 830054
中国食品药品检定研究院，北京 100050 |
Xinjiang Uygur Autonomous Region Drug Inspection and Research Institute NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine (Uyghur) Medicine, Urumqi 830054, China
National Institutes for Food and Drug Control, Beijing 100050, China |
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| 李辉* |
LI Hui |
湖南医药学院 药学院 侗医药研究湖南省重点实验室，湖南 怀化 418000 |
School of Pharmaceutical Sciences, Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua 418000, China |
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| 基金项目:新疆维吾尔自治区2023年度第二批“2+5”重点人才计划项目 |
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| 中文摘要:目的 采用超高效液相色谱-四极杆-静电轨道场离子阱质谱法对复方一枝蒿颗粒的化学成分进行分析。方法 采用Thermo Scientific Syncronis C18色谱柱（100 mm×2.1 mm，1.7 μm），流动相为0.1%甲酸-乙腈，梯度洗脱，流速为0.3 mL·min–1，柱温为45 ℃，进样量为2 μL。在正负离子模式下采集复方一枝蒿颗粒的质谱数据，根据高分辨质谱提供的精确相对分子质量和碎片离子信息，结合对照品比对和相关文献报道对复方一枝蒿颗粒的化学成分进行分析。结果 共分析鉴定出90个化合物，包括2个氨基酸类、8个萜类、25个有机酸类、11个含氮类、44个黄酮类成分。其中，16个成分通过与对照品比对确认。结论 分析鉴定了复方一枝蒿颗粒的化学成分，为其质量控制及药效物质基础研究提供了依据。 |
| 中文关键词:复方一枝蒿颗粒 超高效液相色谱-四极杆-静电轨道场离子阱质谱法 化学成分 |
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| Rapid Identification of Chemical Components in Composite Yizhihao Granules Based on UHPLC-Q-Exactive Orbitrap MS |
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| Abstract:Objective The chemical components in Composite Yizhihao Granules were analyzed using UHPLC-Q-Exactive Orbitrap MS.Methods Thermo Scientific Syncronis C18 column (100 mm×2.1 mm, 1.7 μm) was used with a mobile phase of 0.1% formic acid acetonitrile and a gradient elution at the flow rate of 0.3 mL·min–1. The column temperature was maintained at 45 ℃, and the injection volume was 2 μL. The mass spectrometry data of Composite Yizhihao Granules were collected in positive and negative ion mode. According to the accurate molecular weight and fragment ion information provided by the high-resolution mass spectrometry, the chemical components of Composite Yizhihao Granules were identified and analyzed in combination with the comparison of standard products and relevant literature reports.Results A total of 90 compounds were identified and analyzed, including 2 amino acids, 8 terpenes, 25 organic acids, 11 nitrogen-containing compounds, and 44 flavonoids. Among them, 16 components were confirmed by comparison with the reference standards.Conclusion In this study, the chemical components of Composite Yizhihao Granules were analyzed and identified, providing a basis for its quality control and the study of its pharmacological substances. |
| keywords:Composite Yizhihao Granules UHPLC-Q-Exactive Orbitrap MS chemical components |
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