| UPLC-MS/MS测定心悦胶囊中6个皂苷类成分的含量 |
| 投稿时间：2023-08-02 点此下载全文
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| 引用本文：赵振霞,王晓蕾,耿韫,朱靖,于新兰,刘永利.UPLC-MS/MS测定心悦胶囊中6个皂苷类成分的含量[J].中国现代中药,2024,26(3):528-534 |
| DOI：10.13313/j.issn.1673-4890.20230802002 |
| 摘要点击次数: 95 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 赵振霞 |
ZHAO Zhen-xia |
河北省药品医疗器械检验研究院 河北省中药质量评价与标准研究重点实验室，河北 石家庄 050227 |
Hebei Institute for Drug and Medical Device Control, Hebei Key Laboratory of Traditional Chinese Medicine Quality Evaluation And Standard Research, Shijiazhuang 050227, China |
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| 王晓蕾 |
WANG Xiao-lei |
河北省药品医疗器械检验研究院 河北省中药质量评价与标准研究重点实验室，河北 石家庄 050227 |
Hebei Institute for Drug and Medical Device Control, Hebei Key Laboratory of Traditional Chinese Medicine Quality Evaluation And Standard Research, Shijiazhuang 050227, China |
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| 耿韫 |
GENG Yun |
河北省药品医疗器械检验研究院 河北省中药质量评价与标准研究重点实验室，河北 石家庄 050227 |
Hebei Institute for Drug and Medical Device Control, Hebei Key Laboratory of Traditional Chinese Medicine Quality Evaluation And Standard Research, Shijiazhuang 050227, China |
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| 朱靖 |
ZHU Jing |
河北省药品医疗器械检验研究院 河北省中药质量评价与标准研究重点实验室，河北 石家庄 050227
河北医科大学，河北 石家庄 050017 |
Hebei Institute for Drug and Medical Device Control, Hebei Key Laboratory of Traditional Chinese Medicine Quality Evaluation And Standard Research, Shijiazhuang 050227, China
Hebei Medical University, Shijiazhuang 050017, China |
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| 于新兰* |
YU Xin-lan |
新疆维吾尔自治区药品检验研究院，新疆 乌鲁木齐 830004 |
Xinjiang Institute for Drug Control, Urumqi 830004, China |
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| 刘永利* |
LIU Yong-li |
河北省药品医疗器械检验研究院 河北省中药质量评价与标准研究重点实验室，河北 石家庄 050227 |
Hebei Institute for Drug and Medical Device Control, Hebei Key Laboratory of Traditional Chinese Medicine Quality Evaluation And Standard Research, Shijiazhuang 050227, China |
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| 基金项目:河北省重点研发计划项目（21372502D） |
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| 中文摘要:目的 采用超高效液相色谱-质谱法（UPLC-MS/MS）建立心悦胶囊中主要成分人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1、拟人参皂苷F11、人参皂苷Rd和20(S)-人参皂苷F1的含量测定方法。方法 使用Waters ACQUITY BEH C18色谱柱（100 mm×2.1 mm，1.7 μm），以乙腈-水为流动相，梯度洗脱，流速为0.5 mL·min–1，柱温为40 ℃，电喷雾电离源，多反应离子监测扫描方式进行检测。结果 6个成分分别在质量浓度为9.638~963.800、9.839~983.900、9.951~995.100、5.270~1 054.000、9.608~960.800、4.905~981.000 ng·mL–1时与峰面积线性关系良好（r>0.998），平均加样回收率（RSD）分别为96.9%（2.2%）、100.1%（1.5%）、97.2%（1.8%）、98.5%（2.3%）、96.6%（2.8%）、100.2%（3.5%)。结论 所建立的方法快速、准确、灵敏度高，可用于心悦胶囊中西洋参茎叶总皂苷的质量控制。 |
| 中文关键词:超高效液相色谱-质谱法 心悦胶囊 西洋参茎叶总皂苷 人参皂苷Rg1 人参皂苷Re 人参皂苷Rb1 拟人参皂苷F11 人参皂苷Rd 20（S）-人参皂苷F1 |
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| Simultaneous Determination of Six Saponins in Xinyue Capsules by UPLC-MS/MS |
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| Abstract:Objective To establish the method for determination of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, pseudo-ginsenoside F11, ginsenoside Rd and 20(S)- ginsenoside F1 in Xinyue Capsules by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Methods The analysis was performed on Waters ACQUITY BEH C18 column (100 mm×2.1 mm, 1.7 μm), with acetonitrile (A)-water (B) as the mobile phase, with gradient elution at a flow rate of 0.5 mL·min–1. The column temperature was set at 40 ℃. The analytes were detected using the electrospray ionization (ESI) source in multiple reaction monitoring (MRM) scan mode.Results Six constituents showed good linear relationships within the concentration ranges of 9.638-963.800, 9.839-983.900, 9.951-995.100,5.270-1 054.000,9.608-960.800,4.905-981.000 ng·mL–1 (r>0.998), whose average recoveries (RSD) were 96.9% (2.2%), 100.1% (1.5%), 97.2% (1.8% ), 98.5% (2.3%), 96.6% (2.8%), 100.2% (3.5%), respectively.Conclusion The established method is rapid, accurate, and highly sensitive, making which can be used for quality control of total ginsenosides from Panax quinquefolium stems and leaves in Xinyue Capsules. |
| keywords:UPLC-MS/MS Xinyue Capsules total ginsenoside of Panax quinquefolium stems and leaves ginsenoside Rg1 ginsenoside Re ginsenoside Rb1 pseudo-ginsenoside F11 ginsenoside Rd 20(S)-ginsenoside F1 |
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