| 基于抗氧化谱效关系的肉苁蓉质量标志物研究 |
| 投稿时间：2023-03-18 点此下载全文
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| 引用本文：李莹曼,宋紫腾,侯淇允,张林林,许浚,张铁军.基于抗氧化谱效关系的肉苁蓉质量标志物研究[J].中国现代中药,2024,26(2):265-275 |
| DOI：10.13313/j.issn.1673-4890.20230318001 |
| 摘要点击次数: 151 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 李莹曼 |
LI Ying-man |
天津中医药大学，天津 301617 |
Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China |
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| 宋紫腾 |
SONG Zi-teng |
天津药物研究院 天津市中药质量标志物重点实验室，天津 300462
天津药物研究院 释药技术与药代动力学国家重点实验室，天津 300462 |
Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine, Tianjin Institute of Pharmaceutical Research, Tianjin 300462, China
State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300462, China |
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| 侯淇允 |
HOU Qi-yun |
天津中医药大学，天津 301617 |
Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China |
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| 张林林 |
ZHANG Lin-lin |
天津药物研究院 天津市中药质量标志物重点实验室，天津 300462
天津药物研究院 释药技术与药代动力学国家重点实验室，天津 300462 |
Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine, Tianjin Institute of Pharmaceutical Research, Tianjin 300462, China
State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300462, China |
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| 许浚* |
XU Jun |
天津药物研究院 天津市中药质量标志物重点实验室，天津 300462
天津药物研究院 释药技术与药代动力学国家重点实验室，天津 300462
中国中药协会 药食同源物质评价和利用专业委员会，北京 100061 |
Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine, Tianjin Institute of Pharmaceutical Research, Tianjin 300462, China
State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300462, China
Professional Committee on Evaluation and Utilization of Medicinal and Food Homologous Substances of China Association of Traditional Chinese Medicine, Beijing 100061, China |
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| 张铁军* |
ZHANG Tie-jun |
天津药物研究院 天津市中药质量标志物重点实验室，天津 300462
天津药物研究院 释药技术与药代动力学国家重点实验室，天津 300462 |
Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine, Tianjin Institute of Pharmaceutical Research, Tianjin 300462, China
State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300462, China |
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| 基金项目:国家自然科学基金重点项目（81830111） |
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| 中文摘要:目的 建立基于抗氧化谱效关系的肉苁蓉质量标志物（Q-marker）研究方法，为其药效物质基础及质量控制研究提供依据。方法 采用超高效液相色谱法（UPLC）建立10批肉苁蓉指纹图谱。利用H2O2建立细胞氧化损伤模型，以活性氧自由基半数抑制率、超氧化物歧化酶和过氧化氢酶含量为药效指标测定肉苁蓉抗氧化能力。通过主成分分析、皮尔逊相关性分析和偏最小二乘法-回归分析共同构建谱效关系，并对筛选出的活性成分进行药效验证。结果 建立了肉苁蓉的UPLC指纹图谱，共确定了17个共有峰。基于谱效关联分析筛选出9个成分，活性成分验证结果显示京尼平苷酸、8-表马钱子酸、松果菊苷、毛蕊花糖苷、异毛蕊花糖苷、管花苷A具有抗氧化活性且呈浓度依赖性。结论 基于谱效关系探讨了肉苁蓉的Q-marker，为其药效物质基础及质量标准提升提供参考。 |
| 中文关键词:肉苁蓉 质量标志物 谱效关系 抗氧化 |
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| Quality Markers of Cistanches Herba Based on Antioxidant Spectrum-effect Relationship |
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| Abstract:Objective To establish a research method for the quality markers (Q-markers) of Cistanches Herba (CH) based on the antioxidant spectrum-effect relationship, providing a basis for the study of its pharmacological substances and quality control.Methods Ultra-high-performance liquid chromatography (UPLC) was used to establish fingerprint profiles for 10 batches of CH. A cell oxidative damage model was established using H2O2, and the antioxidant capacity of CH was determined based on the half-maximal inhibitory concentration of reactive oxygen species, as well as the levels of superoxide dismutase and catalase as pharmacological indicators. The spectrum-effect relationship was constructed through principal component analysis, Pearson correlation analysis, and partial least squares regression analysis. The active components were subjected to pharmacological validation.Results Fingerprint profiles for CH were established using UPLC, identifying 17 common peaks. Based on spectrum-effect correlation analysis, nine components were selected. The pharmacological validation results indicated that geniposidic acid, 8-epiloganic acid, echinacoside, acteoside, isoacteoside, and tubuloside A exhibited antioxidant activity in a concentration-dependent manner.Conclusion This study explored Q-markers for CH based on the spectrum-effect relationship, providing reference information for its pharmacological substance foundation and quality standard improvement. |
| keywords:Cistanches Herba quality marker spectrum-effect relationship antioxidant |
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