指纹图谱、多成分定量与模式识别相结合的预知子(三叶木通)配方颗粒质量评价
投稿时间:2024-01-24     点此下载全文
引用本文:曹桂云,宁波,田汝芳,张风超,庄雪松,张京华,林永强,董晓弟,李金鑫,孟兆青.指纹图谱、多成分定量与模式识别相结合的预知子(三叶木通)配方颗粒质量评价[J].中国现代中药,2024,26(6):1059-1067
DOI:10.13313/j.issn.1673-4890.20240124001
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作者中文名作者英文名单位中文名单位英文名E-Mail
曹桂云 CAO Gui-yun 山东宏济堂制药集团股份有限公司,山东 济南 250103 Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan 250103, China  
宁波 NING Bo 山东宏济堂制药集团股份有限公司,山东 济南 250103 Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan 250103, China  
田汝芳 TIAN Ru-fang 山东宏济堂制药集团股份有限公司,山东 济南 250103 Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan 250103, China  
张风超 ZHANG Feng-chao 山东宏济堂制药集团股份有限公司,山东 济南 250103 Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan 250103, China  
庄雪松 ZHUANG Xue-song 山东宏济堂制药集团股份有限公司,山东 济南 250103 Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan 250103, China  
张京华 ZHANG Jing-hua 山东宏济堂制药集团股份有限公司,山东 济南 250103 Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan 250103, China  
林永强 LIN Yong-qiang 山东省食品药品检验研究院,山东 济南 250101 Shandong Provincial Institute for Food and Drug Control, Jinan 250101, China  
董晓弟 DONG Xiao-di 山东宏济堂制药集团股份有限公司,山东 济南 250103 Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan 250103, China  
李金鑫 LI Jin-xin 山东宏济堂制药集团股份有限公司,山东 济南 250103 Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan 250103, China  
孟兆青* MENG Zhao-qing 山东宏济堂制药集团股份有限公司,山东 济南 250103 Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan 250103, China  
基金项目:山东省重点研发订划重大科技创新工程项目(2021CXGC010511);泉城“5150”引才倍增计划项目(2021年)
中文摘要:目的 建立指纹图谱、多成分定量分析和化学模式识别相结合的预知子(三叶木通)配方颗粒质量评价方法。方法 制备预知子(三叶木通)标准汤剂和配方颗粒,建立高效液相色谱法指纹图谱,对其中8个主要成分含量进行测定,并计算转移率和出膏率。进行相似度评价、聚类分析(HCA)、主成分分析(PCA)、偏最小二乘法-判别分析(PLS-DA),评价配方颗粒和标准汤剂化学成分的一致性。结果 预知子(三叶木通)标准汤剂中新绿原酸、绿原酸、隐绿原酸、木通苯乙醇苷B、异绿原酸B、异绿原酸A、异绿原酸C、木通皂苷D质量分数分别为1.94~10.33、2.18~13.77、1.77~9.15、2.31~19.34、1.77~6.48、1.16~5.55、2.55~9.90、0.92~8.93 mg·g–1,转移率分别为36.25%~56.02%、24.64%~40.23%、35.88%~58.41%、29.45%~50.69%、16.55%~29.54%、26.25%~46.59%、25.33%~41.66%、11.03%~17.35%,出膏率为13.59%~24.49%。预知子(三叶木通)配方颗粒指标成分含量、转移率、出膏率与标准汤剂接近,指纹图谱相似度、HCA、PCA、PLS-DA评价表明,配方颗粒和标准汤剂化学成分一致性较好。结论 预知子(三叶木通)配方颗粒与标准汤剂化学成分一致性较好,结果合理、可靠,可为其工艺优化及质量评价提供参考。
中文关键词:三叶木通  标准汤剂  配方颗粒  指纹图谱  多成分定量分析  化学模式识别
 
Quality Evaluation of Akebiae Frucus [Akebia trifoliate (Thunb.) Koidz.] Dispensing Granules by Combination of Fingerprint, Multi-Component Quantitative Analysis, and Chemical Pattern Recognition
Abstract:Objective To establish a quality evaluation method for Akebiae Frucus [A. trifoliate (Thunb.) Koidz.] dispensing granules by combining fingerprinting, multi-component quantitative analysis, and chemical pattern recognition.Methods Standard Akebiae Frucus [A. trifoliate (Thunb.) Koidz.] decoctions and dispensing granules were prepared. High-performance liquid chromatography (HPLC) fingerprinting was established, and the contents of eight components were determined. The transfer rate and extraction rate were calculated, and similarity evaluation, cluster analysis (HCA), principal component analysis (PCA), and partial least squares discriminant (PLS-DA) were performed to evaluate the consistency of chemical components between dispensing granules and standard decoctions.Results The mass fractions of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, calceolarioside B, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, and akebia saponin D in standard Akebiae Frucus [A. trifoliate (Thunb.) Koidz.] decoctions were 1.94-10.33, 2.18-13.77, 1.77-9.15, 2.31-19.34, 1.77-6.48, 1.16-5.55, 2.55-9.90, and 0.92-8.93 mg·g–1, respectively. The transfer rates of these components were 36.25%-56.02%, 24.64%-40.23%, 35.88%-58.41%, 29.45%-50.69%, 16.55%-29.54%, 26.25%-46.59%, 25.33%-41.66%, and 11.03%-17.35%. The extraction rate was 13.59%-24.49%. The component content, transfer rate, and extraction rate of Akebiae Frucus [A. trifoliate (Thunb.) Koidz.] dispensing granules were close to those of standard decoctions. The results of similarity evaluation, HCA, PCA, and PLS-DA indicated good consistency of chemical components between dispensing granules and standard decoctions.Conclusion There was good consistency between dispensing granules and standard decoctions of Akebiae Frucus [A. trifoliate (Thunb.) Koidz.]. The results were reasonable and reliable, which can provide a reference for process optimization and quality evaluation.
keywords:Akebia trifoliate (Thunb.) Koidz.  standard decoctions  dispensing granules  fingerprint  multi-component quantitative analysis  chemical pattern recognition
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