败酱草及其近缘种叶绿体全基因组分析和DNA条形码的构建
投稿时间:2024-05-21  修订日期:2024-06-05   点此下载全文
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作者中文名作者英文名单位中文名单位英文名E-Mail
孟文娜 mengwenna 安徽医科大学 Anhui Medical University 2419187824@qq.com 
浦香东 puxiangdong 安徽医科大学 Anhui Medical University xdpu@ahmu.edu.cn 
蒲婧哲 pujingzhe 安徽省食品药品检验研究院 Anhui Institute for Food and Drug Control pujingzhe@163.com 
申传璞 shengchuanpu 安徽医科大学 Anhui Medical University shenchuanpu@126.com 
陈庆 chenqing 亳州济人药业 Anhui Jiren Pharmaceutical Co., Ltd. 13592542608@163.com 
张磊 zhanglei 安徽医科大学 Anhui Medical University zhangl@ahmu.edu.cn 
吕雄文 lvxiongwen 安徽医科大学 Anhui Medical University lxw31288@aliyun.com 
马陶陶* mataotao 安徽医科大学 Anhui Medical University mataotao@ahmu.edu.cn 
基金项目:国家药品监督管理局中药质量研究与评价重点实验室开放课题(AHYJ-KFKT-202103)
中文摘要:目的:基于败酱药材基原植物的叶绿体基因组序列分析开发特定的DNA条形码,准确鉴别败酱草及近缘种。方法:采用Illumina高通量测序技术对败酱科败酱属植物白花败酱P. villosa Juss、黄花败酱P. scabiosifolia Fisch和异叶败酱P. heterophylla Bunge的叶绿体基因组进行测序。使用生物信息学软件对基因组进行组装注释、特征分析、序列比较和系统发育分析。基于叶绿体基因组高突变区构建特异性DNA条形码进行验证。结果:三种败酱属植物叶绿体基因组呈四分体结构,全长分别为159 585 bp、158 919 bp、158 919 bp,编码130、132、132个基因,包含8个rRNA基因和37个tRNA基因,蛋白质编码基因为85、87、87个。ccsA、ndhG、rpl23、ndhF序列可作为特异DNA条形码成功扩增16份样品,将黄花败酱、白花败酱和少蕊败酱聚类在同分支,异叶败酱和糙叶败酱聚类在同一支。ccsA、ndhG和ndhF序列可进一步鉴别出糙叶败酱和异叶败酱。结论:ccsA、ndhG、rpl23和ndhF序列可作为补充特异DNA条形码将败酱草及近缘物种区分开,为实现败酱草及其近缘种的分类提供了依据。
中文关键词:败酱草  叶绿体基因组  分子鉴定  DNA条形码  系统发育分析
 
Chloroplast Whole Genome Analysis and DNA Barcode Construction of Patrinia scabiosaefolia and Its Related Species
Abstract:Objective:Develop specific DNA barcodes based on the chloroplast genome sequence analysis of the original plants of Patrinia medicinal materials to accurately identify Patrinia scabiosaefolia and related species. Methods:The chloroplast genomes of the plants of patrinia, including Patrinia villosa, P.scabiosaefolia and P.heterophylla were sequenced using Illumina high-throughput sequencing technology. Bioinformatics software was employed for genome assembly, annotation, feature analysis, sequence comparison, and phylogenetic analysis. High mutation regions in the chloroplast genome were identified, and specific DNA barcodes were constructed for validation. Results:The chloroplast genomes of three species of Patrinia plants exhibit a tetrad structure, with a total length of 159 585 bp, 158 919 bp, and 158 919 bp, respectively. They encode 130, 132, and 132 genes, including 8 rRNA genes and 37 tRNA genes. The protein coding genes are 85, 87, and 87. ccsA, ndhG, rpl23, and ndhF primers can be used as specific DNA barcodes to successfully amplify 16 samples, accurately clustering Patrinia scabiosaefolia, Patrinia villosa, and Patrinia monandra were clustered in the same branch, while Patrinia heterophylla Bunge and Patrinia scabra Bunge were clustered in a different branch.The ccsA, ndhG, and ndhF sequences can further distinguish Patrinia heterophylla Bunge from Patrinia scabra Bunge. Conclusion:The ccsA, ndhG, rpl23, and ndhF sequences can serve as supplementary specific DNA barcodes to distinguish Patrinia scabiosaefolia and related species, providing a basis for the classification of Patrinia scabiosaefolia and its close relatives.
keywords:Patrinia scabiosaefolia  chloroplast genome  molecular identification  DNA barcode  phylogenetic analysis
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