| 海南裸花紫珠药材中7种成分含量分析 |
| 投稿时间：2017-09-01 点此下载全文
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| 引用本文：谭湘杰,于福来,庞玉新,黄梅,陈振夏,杨全,李伟.海南裸花紫珠药材中7种成分含量分析[J].中国现代中药,2018,20(2):173-178 |
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| 基金项目:海南省重大科技计划（ZDKJ2016005-03） |
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| 中文摘要:目的：建立同时测定裸花紫珠药材中7种成分含量的方法，并对海南不同来源的裸花紫珠药材样品中7种成分含量进行分析。方法：采用HPLC，色谱柱为Agilent-TC C18，流动相为乙腈-0.4%磷酸（梯度洗脱），流速为1 mL·min-1，波长为330 nm，柱温为30 ℃。通过方差分析和主成分分析比较海南不同来源裸花紫珠含量差异。结果：该色谱条件下7种成分分离程度良好，在规定的线性范围内有较好的线性关系（r≥0.999 0），平均加样回收率在96.4%～105.5%；来自海南的75份裸花紫珠药材样品中7个成分含量差异明显，变异系数在33.99%～83.91%，不同气候条件下具有统计学差异（P＜0.05）。结论：该方法简便可行，重复性好，可用于裸花紫珠的质量控制；裸花紫珠药材主要化学成分积累受产地气候因素影响较大。 |
| 中文关键词:裸花紫珠 7种成分 HPLC测定 产地 气候区 |
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| Analysis of Seven Components in Callicarpa nudiflora from Hainan Province |
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| Abstract:Objective：To establish an HPLC method for the simultaneous determination of seven components and analyze the content variation of Callicarpa nudiflora in different origins.Methods：Analysis was performed on an Agilent TC-C18 column（250 mm×4.6 mm，5 μm）eluted with acetonitrile（A）and 0.4% phosphoric acid（B）in a gradient program.The flow rate was 1 mL·min-1，the detection wavelength was 330 nm，and the column temperature was 30 ℃，the ANOVA and PCA were used to analyze the content variance of C.nudiflora from different origins in Hainan province.Results：Under this chromatographic condition，the seven components showed good separation degree and regression（r＞0.999 0）with linear ranges，their recoveries were in the range of 96.4%-105.5%.Obvious differences was existed in the seven components of 75 samples of C.nudiflora from Hainan province with CVs were in the range of 33.99%-83.91%，the seven component of C.nudiflora which under different climatic conditions was significantly different（P＜0.05）.Conclusion：The method is simple，accurate，and can be used for the quality control of C.nudiflora.The main chemical components accumulation of C.nudiflora.were greatly influenced by climatic conditions in place of origin. |
| keywords:Callicarpa nudiflora Hook.et Arn seven components HPLC-determination origin climatic region |
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