基于丹参基因组的蛋白磷酸酶2C家族的系统分析
投稿时间:2018-03-12     点此下载全文
引用本文:徐志超,浦香东,宋经元.基于丹参基因组的蛋白磷酸酶2C家族的系统分析[J].中国现代中药,2018,20(6):652-657
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作者中文名作者英文名单位中文名单位英文名E-Mail
徐志超 XU Zhi-chao 中国医学科学院 北京协和医学院 药用植物研究所/国家中医药管理局 中药资源保护重点研究室,北京100193 Chinese Academy of Medical Sciences,Peking Union Medical College,Institute of Medicinal Plant Development
Key Lab of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People’s Republic of China,Beijing 100193,China
 
浦香东 PU Xiang-dong 中国医学科学院 北京协和医学院 药用植物研究所/国家中医药管理局 中药资源保护重点研究室,北京100193 Chinese Academy of Medical Sciences,Peking Union Medical College,Institute of Medicinal Plant Development
Key Lab of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People’s Republic of China,Beijing 100193,China
 
宋经元 SONG Jing-yuan 中国医学科学院 北京协和医学院 药用植物研究所/国家中医药管理局 中药资源保护重点研究室,北京100193 Chinese Academy of Medical Sciences,Peking Union Medical College,Institute of Medicinal Plant Development
Key Lab of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People’s Republic of China,Beijing 100193,China
宋经元,研究员,研究方向:本草基因组学;E-mail:jysong@implad.ac.cn 
基金项目:国家自然科学基金青年科学基金(31700264)
中文摘要:目的:基于全基因组策略系统解析药用植物丹参的蛋白磷酸酶2C家族成员,为研究丹参逆境胁迫响应的分子机制奠定基础。方法:采用BLASTP序列比对、系统发育树构建、基因表达分析等方法解析丹参PP2C亚家族成员、基因结构以及差异表达图谱。结果:丹参基因组共注释到83个PP2C家族成员,在高等植物中保守存在;系统进化分析将PP2C家族分为13个亚家族(PP2CA-PP2CL,A、B、C、D、E、F1、F2、G、H、I、J、K、L),结构域高度保守;PP2CA亚家族基因启动子含有多种逆境胁迫应答的顺式作用元件,大部分PP2CA基因表达显著响应逆境胁迫信号。结论:本实验系统研究丹参PP2C基因的结构、在不同组织部位的表达及逆境胁迫下的响应,为揭示丹参响应生物或非生物胁迫的分子机制研究提供理论依据。
中文关键词:丹参  蛋白磷酸酶  PP2C  逆境胁迫
 
Genome-wide Characterization of PP2C Gene Family in Salvia miltiorrhiza
Abstract:Objective:To systematically identify the protein phosphatases 2C(PP2C)family of Salvia miltiorrhiza based on genome-wide strategy.Methods:The family members of PP2C genes,structures,and expression profile were identified by BLSTP,phylogenetic tree,and different gene expression analysis.Results:In total,83 PP2C genes were identified in S.miltiorrhiza,highly conserved in angiosperm.The PP2C family were phylogenetically divided into 13 subfamilies,including PP2CA,B,C,D,E,F1,F2,G,H,I,J,K,L subfamilies.The promoters of PP2CA subfamily included a series of cis-acting elements,and most of gene expression were significantly responded to stress.Conclusion:These results provided a basis for analyzing the evolutional characterization of PP2C family,and for studying the molecular mechanism of PP2C genes under varies stress response.
keywords:Salvia miltiorrhiza  protein phosphatases  PP2C  stress response
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