欢迎访问中国现代中药网站

作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
蒋洪亮	JIANG Hong-liang	贵州大学药学院，贵州贵阳550025	School of Pharmaceutical Sciences，Guizhou University，Guiyang 550025，China
麻浩	MA Hao	军事科学院军事医学研究院毒物药物研究所，北京100850	Institute of Pharmacology and Toxicology，Institute of Military Medicine，Academy of Military Sciences，Beijing 100850，China
何丽芳	HE Li-fang	军事科学院军事医学研究院毒物药物研究所，北京100850	Institute of Pharmacology and Toxicology，Institute of Military Medicine，Academy of Military Sciences，Beijing 100850，China
赵修南	ZHAO Xiu-nan	军事科学院军事医学研究院毒物药物研究所，北京100850	Institute of Pharmacology and Toxicology，Institute of Military Medicine，Academy of Military Sciences，Beijing 100850，China
单俊杰	SHAN Jun-jie	军事科学院军事医学研究院毒物药物研究所，北京100850	Institute of Pharmacology and Toxicology，Institute of Military Medicine，Academy of Military Sciences，Beijing 100850，China	单俊杰，研究员，研究方向：中药多糖化学及生物活性; E-mail:shanjunjie0012@126.com
王海南	WANG Hai-nan	贵州大学药学院，贵州贵阳550025	School of Pharmaceutical Sciences，Guizhou University，Guiyang 550025，China	王海南，研究员，研究方向：药物化学；E-mail：md_wanghainan@126.com

中文摘要:目的：将发酵虫草菌粉（Cs-4）粗多糖进行分离纯化，并观察纯化产物对ConA抑制人正常肝细胞（L-O2细胞株）增殖活性的拮抗作用。方法：发酵虫草菌粉（Cs-4）通过水提醇沉、复溶、透析、浓缩、冷冻干燥得粗多糖，采用DEAE-纤维素离子交换、sephadex-G-100柱色谱分离纯化粗多糖，MTT法检测细胞增殖活性。结果：发酵虫草菌粉（Cs-4）粗多糖经分离纯化得到均一成分，命名为CSMP-60-B-I。高效凝胶渗透色谱法测得其相对峰高分子量为32 336 Da。毛细管电泳测得单糖组成为木糖-阿拉伯糖-葡萄糖-半乳糖-甘露糖（0.09∶0.20∶1.00∶3.70∶3.58）。高浓度ConA能抑制L-O2细胞增殖，而加入CSMP-60-B-I后，这种抑制作用大大减弱。结论：发酵虫草菌粉（Cs-4）粗多糖的分离纯化产物CSMP-60-B-I对ConA抑制L-O2细胞增殖活性有拮抗作用，提示其可能有保肝作用。

中文关键词:发酵虫草菌粉多糖分离纯化肝细胞

Physicochemical Properties of Homogeneous Polysaccharide Extracted from Fermented Cordyceps Powder and It’s Antagonism of Inhibition Effect on Cell Proliferation Activity of L-O2

Abstract:Objective：To isolate and purify the total polysaccharides extracted from fermented Cordyceps powder（Cs-4）and observe the antagonism of inhibition effect on cell proliferation activity of L-O2.Methods：The fermented Cordyceps powder（Cs-4）was extracted with water at 60 ℃ and precipitated with ethanol，then redissolved，dialyzed，concentrated and lyophilized to obtain the total polysaccharides.The total polysaccharides were fractionated by a DEAE-cellulose column.The cell proliferation activity of L-O2 was detected by MTT.Results：The total polysaccharides extracted from fermented Cordyceps powder（Cs-4）were fractionated to obtain homogeneous polysaccharide named CSMP-60-B-I.The relative peak molecular weight of CSMP-60-B-I was 32 336 Da，determined by high performance gel permeation chromatography.The composition of monosaccharides and molar ratios were xylose-arabinose-glucose-galactose-mannose（0.09:0.20:1.00:3.70:3.58），determined by capillary electrophoresis.The results showed that ConA in high concentration could inhibit the cell proliferation activity of L-O2 in vitro.After the addition of CSMP-60-B-I，the inhibiting effect was greatly reduced.Conclusion：CSMP-60-B-I may have the effect of reducing inhibition of cell proliferation activity of L-O2by ConA in high concentration，implicating that CSMP-60-B-I might have hepatoprotective effect.

keywords:Fermented Cordyceps powder polysaccharide isolation and purification hepatocyte

查看全文查看/发表评论下载PDF阅读器