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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
曾诚	Zeng Cheng	中兽药湖南省重点实验室，湖南长沙410128	Hunan Key Laboratory of Chinese Veterinary Medicine，Changsha，Hunan 410128，China
曾建国	Zeng Jian-guo	中兽药湖南省重点实验室，湖南长沙410128	Hunan Key Laboratory of Chinese Veterinary Medicine，Changsha，Hunan 410128，China	曾建国，教授，研究方向：中药资源与中兽药创制等综合利用；Tel：（0731）84673824，E-mail：zengjianguo@hunau.edu.cn

基金项目:中药材全产业链发展（2016SK3002）。

中文摘要:目的：建立测定博落回茎叶中非生物碱N-对香豆酰酪胺含量的方法，为博落回茎叶微粉兽药质量控制提供新的评价指标。方法：采用HPLC，色谱柱为资生堂MG3 C18（250 mm×4.6 mm，5 μm）；流动相为甲醇∶水（40∶60）梯度洗脱［1］；检测波长为290 nm；流速为1.0 mL·min-1；柱温为35 ℃。结果：N-对香豆酰酪胺的含量在0.077 5~0.470 4 mg（r=0.999 6）与峰面积呈良好的线性关系，该测定N-对香豆酰酪胺含量方法的平均回收率为99.45%。结论：所建立的方法操作简单，方便快捷，重复性好，适合用于测定博落回茎叶中N-对香豆酰酪胺的含量。

中文关键词:博落回 N-对香豆酰酪胺高效液相色谱法含量测定

Content Determination of N-p-Coumaroyltyramine in Stems and Leaves of Macleaya cordata from Different Place of Production by HPLC

Abstract:Objective：To establish a method for the determination of non-alkaloid N-p-Coumaroyltyramine in stems and leaves of Macleaya cordata.This study provided a new evaluation index for the quality control of micropowder veterinary drugs in the stems and leaves of M.cordata.Methods：HPLC method was used in this paper，the chromatographic column was Shiseido MG3 C18（250 mm×4.6 mm，5 μm），the mobile phase was methanol∶water（40∶60）gradient elution ［1］，the detection wavelength was 290 nm，the flow rate was 1.0 mL·min-1，and the column temperature was 35℃.Results：The content of N-p-coumaroyltyramine ranged from 0.077 5 mg to 0.470 4 mg（r=0.999 4），which showed a good linear relationship with the peak area.The average recovery of the method for the determination of N-p-coumaroyltyramine was 99.45%.Conclusion：The method is simplicity of operator，convenient and reproducible.It is suitable for the determination of N-p-coumaroyltyramine in the stems and leaves of M.cordata.

keywords:Macleaya cordata N-p-coumaroyltyramine HPLC content determination

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