| 外源NO对桔梗幼苗生长和总皂苷积累的影响 |
| 投稿时间：2019-01-01 点此下载全文
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| 引用本文：张坚,李先宽,李兴林,马琳,陈志娟.外源NO对桔梗幼苗生长和总皂苷积累的影响[J].中国现代中药,2019,21(10):1402-1406 |
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| 基金项目:国家自然科学基金项目（U1332123）；中央本级重大增减支项目（2060302） |
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| 中文摘要:目的：考察外源一氧化氮（nitric oxide，NO）对桔梗幼苗生长和次生代谢产物总皂苷类成分积累的影响。方法：培养桔梗实生苗，然后利用喷灌法连续1周喷洒不同浓度梯度的NO供体硝普钠（sodium nitroprusside，SNP）溶液，记录幼苗的生长状况，并对桔梗总皂苷、脯氨酸（proline，Pro）、丙二醛（malondialdehyde，MDA）、超氧化物歧化酶（superoxide dismutase，SOD）、过氧化物酶（peroxidase，POD）、过氧化氢酶（catalase，CAT）等生理指标进行测定。结果：随SNP度增加，桔梗幼苗生长、皂苷积累和生理指标多数呈现先增加后降低的趋势，MDA含量则呈现先降低后增加的趋势；低浓度SNP有助于促进桔梗的生长，并促进其总皂苷成分积累。结论：低浓度的外源NO对桔梗幼苗的生长有一定的促进作用，且可明显提高幼苗中总皂苷含量。 |
| 中文关键词:桔梗 硝普钠 总皂苷 |
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| The Effects of Exogenous NO on the Seedlings Growth and Accumulation of Secondary Metabolism of Platycodon grandiflorum |
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| Abstract:Objective：To investigate the effect of exogenous NO（nitric oxide）on Platycodon grandiflorum seedling growth and the total saponins of secondary metabolites accumulation.Methods：Platycodon grandiflorum seedlings were cultivated，and then，a certain concentration gradient of SNP（sodium nitroprusside），a nitric oxide donor，were sprayed by leaf spraying method for a week.Recorded the situation of seedling growth，and measured the physiological indexs of the total saponins of Platycodon grandiflorum，proline，MDA，SOD，POD and CAT.Results：With the increase of the concentration of SNP，the seedling growth，the saponin accumulation and the majority of physiological indexes presented first increase and then decrease trend.MDA content showed the trend of decreasing first and then increasing.Low concentrations of SNP helped to promote the seedling growth and the total saponin accumulation.Conclusion：Low concentrations of exogenous NO promote the seedling growth of Platycodon grandiflorum and can significantly increase the saponin content. |
| keywords:Platycodon Grandiflorum sodium nitroprusside（SNP） total saponins |
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