| 茵陈饮片与茵陈标准汤剂的质量比较研究 |
| 投稿时间：2019-03-15 点此下载全文
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| 引用本文：田芳,阮群珈,石星,吴孟华,张英,曹晖,马志国.茵陈饮片与茵陈标准汤剂的质量比较研究[J].中国现代中药,2020,22(1):98-102 |
| DOI： |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 田芳 |
TIAN Fang |
暨南大学 岭南传统中药研究中心，广东广州510632 |
Research Center for TCM of Lingnan (Southern China),Jinan University,Guangzhou 510632,China |
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| 阮群珈 |
RUAN Qun-jia |
暨南大学 药学院，广东广州510632 |
College of Pharmacy,Jinan University,Guangzhou 510632,China |
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| 石星 |
SHI Xing |
乌鲁木齐质量计量检测研究院，新疆乌鲁木齐830000 |
Urumqi Academy of Quality and Metrology Inspection，Urumqi,830000，China |
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| 吴孟华 |
WU Meng-hua |
暨南大学 岭南传统中药研究中心，广东广州510632 |
Research Center for TCM of Lingnan (Southern China),Jinan University,Guangzhou 510632,China |
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| 张英 |
ZHANG Ying |
暨南大学 岭南传统中药研究中心，广东广州510632 |
Research Center for TCM of Lingnan (Southern China),Jinan University,Guangzhou 510632,China |
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| 曹晖 |
CAO Hui |
暨南大学 岭南传统中药研究中心，广东广州510632 |
Research Center for TCM of Lingnan (Southern China),Jinan University,Guangzhou 510632,China |
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| 马志国 |
MA Zhi-guo |
暨南大学 岭南传统中药研究中心，广东广州510632 |
Research Center for TCM of Lingnan (Southern China),Jinan University,Guangzhou 510632,China |
马志国，副教授，研究方向：中药饮片质量研究；Tel：（020）85223784，E-mail：mzg79@hotmail.com |
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| 基金项目:国家重点研发计划（2019YFC1711500） |
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| 中文摘要:目的：比较茵陈饮片与茵陈标准汤剂的质量。方法：按照标准汤剂制备要求，制备12批茵陈饮片标准汤剂，测定绿原酸含量，计算其转移率，测定汤剂的出膏率，并建立茵陈饮片与标准汤剂的HPLC指纹图谱，以0.1%甲酸水（A）-乙腈（B）为流动相，梯度洗脱，柱温为30 ℃，流速为1 mL·min-1，检测波长为330 nm。结果：12批茵陈饮片标准汤剂的绿原酸转移率为31.0%～62.4%，出膏率21.2%～30.1%，茵陈饮片与标准汤剂指纹图谱相似度均>0.96，指认了7个共有峰，分别为新绿原酸、绿原酸、隐绿原酸、咖啡酸、1，3-二咖啡酰奎宁酸、3，4-二咖啡酰奎宁酸、4，5-二咖啡酰奎宁酸。结论：该研究中茵陈饮片标准汤剂制备方法规范，指纹图谱相似度高，其方法精密度、稳定性和重复性良好，可为茵陈饮片标准汤剂质量控制提供参考。 |
| 中文关键词:茵陈 标准汤剂 绿原酸 指纹图谱 质量评价 |
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| Comparative Study on the Quality of Artemisia capillaris and Its Standard Decoction |
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| Abstract:Objective：Compare the quality of Artemisia capillaris and standard decoction. Methods：12 batches of Artemisia capillaris standard decoctions were prepared following the preparation conditions of the TCM standard decoction. The transfer rate of chlorogenic acid and extract rate were calculated. HPLC fingerprints of Artemisia capillaris and its standard decoction were established under a flow rate of 1.0 mL·min-1 and eluted with a mobile phase of acetonitrile (B)-0.1% formic acid acid solution (A) in a gradient mode. The column temperature was set at 30 ℃ and the detection wavelength was 330 nm. Results：the chlorogenic acid transfer rate of standard decoctions was at a range of 31.0% to 62.4%，the extract rate was from 21.2% to 30.1%. The fingerprint similarities of Artemisia capillaris and its standard decoction were all greater than 0.96，and seven were identified，namely，neochlorogenic acid and chlorogenic acid. cryptochlorogenic acid，caffeic acid，1，3-dicaffeoylquinic acid，3，4-dicaffeoylquinic acid and 4，5-dicaffeoylquinic acid. Conclusion：In this study，the preparation method for Artemisia capillaris decoction was standard，with high similarity in fingerprint，and showing high precision，stability and repeatability in fingerprint analysis. Thus，this study can provide a reference for the quality control of Artemisia capillaris standard decoction. |
| keywords:Artemisia capillaris standard decoction chlorogenic acid fingerprint quality evaluation |
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