| 香茯健脾苦荞茶的制备工艺及其含量测定 |
| 投稿时间：2019-11-04 点此下载全文
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| 引用本文：孙芮芮,李明杰,李慧峰,裴妙荣.香茯健脾苦荞茶的制备工艺及其含量测定[J].中国现代中药,2020,22(12):2085-2089 |
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| 基金项目:晋药综合开发利用协同创新中心项目（2017-JYXT-02，2017-JYXT-14） |
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| 中文摘要:目的：研究香茯健脾苦荞茶的最佳提取、制备工艺并建立该茶剂中芦丁含量测定的方法。方法：采用单因素及正交试验设计分别对香薷挥发油提取率及香薷、茯苓水提工艺进行优选，从而得出最佳提取工艺。将提取药液吸附于苦荞上，判断其最佳吸附比。采用HPLC测定香茯健脾苦荞茶中芦丁含量。Diamonsil C18（200 mm×4.6 mm，5 μm）色谱柱；流动相为甲醇-0.5%冰乙酸（34∶66）；流速为1.0 mL·min-1；柱温为30 ℃；检测波长为256 nm。结果：香薷挥发油提取率的最佳工艺为料液比1∶12，浸泡2.0 h，提取4.0 h；香苓健脾苦荞茶最佳提取工艺为料液比1∶10，提取时间为0.5 h，提取次数为3次；浓缩药液与苦荞的最佳吸附比1∶1；香茯健脾苦荞茶所含芦丁质量分数为3.47 mg·g-1，苦荞的有效成分芦丁的线性关系良好（r＞0.999 9）。结论：此提取工艺简单经济、吸附比例合理、成型工艺可行；所建立的含量测定方法简单、准确、灵敏、重复性好，可为香茯健脾苦荞茶的进一步开发、品质评价和质量控制提供参考。 |
| 中文关键词:香薷 茯苓 提取工艺 制备工艺 芦丁 |
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| Study on Preparation Technology and Content Determination of Xiangfu Jianpi Tartary Buckwheat Tea |
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| Abstract:Objective：The optimum extraction and preparation technology of Xiangfu Jianpi tartary buckwheat tea was studied，and a method for the determination of rutin in the tea was established. Methods：The extraction rate of volatile oil from Mosla chinensis and the water extraction process of M.chinensis and Poria cocos were optimized respectively by single factor and orthogonal test，and the best extraction process was obtained. The extraction solution was adsorbed on tartary buckwheat to judge the best adsorption ratio. The content of rutin in Xiangfu Jianpi tartary buckwheat tea was determined by HPLC with a conditions as following：column：diamonsil C18 （200 mm×4.6 mm，5 μm）；mobile phase：methanol-0.5% glacial acetic acid （34∶66）；flow rate：1.0 mL·min-1；column temperature：30 ℃；detection wavelength：256 nm. Results：The optimum extraction process of volatile oil in M.chinensis was 1∶12，2.0 h for soaking and 4.0 h for extraction；1∶10 for Xiangfu Jianpi tartary buckwheat tea，0.5 h for extraction and 3 times for extraction （r＞0.999 9）. Conclusion：The extraction process of this experimental study is simple and economical，the adsorption ratio is reasonable，and the molding process is feasible. The content determination method established in this study is simple，accurate，sensitive and reproducible，which provides a scientific basis for the further development，quality evaluation and quality control of Xiangfu Jianpi tartary buckwheat tea. |
| keywords:Mosla chinensis Poria cocos extraction process preparation process rutin |
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