| 基于HPLC指纹图谱的白芍及酒白芍比较分析 |
| 投稿时间：2020-07-18 点此下载全文
|
| 引用本文：杨艳玲,刘彩凤,黄嘉怡,杨海菊,李花花,杜守颖,白洁.基于HPLC指纹图谱的白芍及酒白芍比较分析[J].中国现代中药,2021,23(8):1374-1379 |
| DOI：10.13313/j.issn.1673-4890.20200718002 |
| 摘要点击次数: 462 |
| 全文下载次数: 144 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 杨艳玲 |
YANG Yan-ling |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
|
| 刘彩凤 |
LIU Cai-feng |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
|
| 黄嘉怡 |
HUANG Jia-yi |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
|
| 杨海菊 |
YANG Hai-ju |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
|
| 李花花 |
LI Hua-hua |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
|
| 杜守颖 |
DU Shou-ying |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
|
| 白洁 |
BAI Jie |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
|
|
| 基金项目:中央高校基本科研业务费专项资金（2020-JYB-ZDGG-031） |
|
| 中文摘要:目的 建立白芍及酒白芍的高效液相色谱法（HPLC）指纹图谱，结合出膏率和指标性成分含量测定，评价白芍炮制前后的质量属性。方法 采用HPLC，以InertSustainSwiftTM C18（250 mm×4.6 mm，5 μm）为色谱柱，乙腈-0.1%磷酸溶液为流动相梯度洗脱，检测波长为230 nm，柱温为30 ℃，体积流量为1.0 mL·min–1，进样量为15 μL，分别建立白芍药材及炮制品酒白芍的指纹图谱；采用HPLC测定炮制前后样品中芍药苷的含量；运用指纹图谱相似度评价方法，结合出膏率及指标性成分含量测定对白芍和酒白芍进行比较研究。结果 建立了白芍及酒白芍的HPLC指纹图谱，标定了30批次样品的8个共有峰。15批白芍及15批酒白芍与各自的对照图谱比较相似度分别为0.988~0.999和0.991~0.999，生品与炮制品比较相似度达0.999。炮制前后出膏率平均变化幅度为2.45%。含量测定结果表明，炮制前后芍药苷质量分数变化为–2.13%~0.49%。结论 建立的方法可对白芍及酒白芍的质量属性进行有效评价，具有一定的应用价值。 |
| 中文关键词:白芍 酒白芍 指纹图谱 相似度评价 |
| |
| Comparative Analysis of Paeoniae Radix Alba and Rice Wine-processed Paeoniae Radix Alba Based on HPLC Fingerprint |
|
|
| Abstract:Objective To establish the high performance liquid chromatography (HPLC) fingerprints for Paeoniae Radix Alba (PRA) and rice wine-processed Paeoniae Radix Alba (PPRA) and to evaluate the quality of PRA before and after processing based on extraction rate and content of index component.Methods HPLC conditions are as follows: InertSustainSwiftTM C18 column (250 mm×4.6 mm, 5 μm), column temperature of 30 ℃, gradient elution with acetonitrile-0.1% phosphoric acid solution, flow rate of 1.0 mL·min–1, injection volume of 15 μL, and detection wavelength of 230 nm. The fingerprints of PRA and PPRA were respectively established and compared and the content of paeoniflorin in the two kinds of samples was determined by HPLC. Based on the similarity of fingerprint, extraction rate, and index component content, the quality of PRA and PPRA was compared.Results According to the HPLC fingerprints, the 15 batches of PRA and 15 batches of PPRA separately shared 8 peaks. The similarities of PRA fingerprints to the reference fingerprints were in the range of 0.988-0.999 and the corresponding range for PPRA was 0.991-0.999. The similarity in fingerprint between the raw and processed products was 0.999. The average variation of extraction rate after processing was 2.45% and the variation of the paeoniflorin content was –2.13%-0.49%.Conclusion The established method can effectively reflect the quality properties of PRA and PPRA, which is of certain application value. |
| keywords:Paeoniae Radix Alba rice wine-processed Paeoniae Radix Alba fingerprint similarity evaluation |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
